Phlebotomy ASCP

American Society for Clinical Pathology phlebotomy certification.

Introduction to PhlebotomySafety and Infection ControlBlood Collection Methods
Order of Draw and Tube TypesPatient Identification and Special PopulationsComplications and Troubleshooting
Preparing for the ASCP ExamProfessionalism and CommunicationQuality Assurance and Documentation
Time Management for Test DayActive Recall and Practice
Introduction to Phle...Safety and Infection...Blood Collection Met...Order of Draw and Tu...Patient Identificati...Complications and Tr...Preparing for the AS...Professionalism and ...Quality Assurance an...Time Management for ...Active Recall and Pr...
Advanced Topics

Order of Draw and Tube Types

Why Order of Draw Matters

The order in which blood collection tubes are filled is crucial to prevent cross-contamination of additives between tubes.

The Standard Order

  1. Blood culture bottles
  2. Light blue (citrate)
  3. Red or gold (serum)
  4. Green (heparin)
  5. Lavender (EDTA)
  6. Gray (fluoride)

Tube Additives

Each tube contains a specific additive (or none) to prepare the blood for different tests. Using the incorrect order can affect test results.

Memory Tricks

Try using mnemonics like “Boys Love Ravishing Girls Like Dieters” to remember the sequence!

Real-World Relevance

Proper tube selection and order prevent erroneous lab results and avoid costly repeat tests.

Examples

  • A phlebotomist draws a lavender tube before a gray tube to prevent contamination of glucose samples.

  • Blood cultures are always collected first to avoid skin contaminants.

In a Nutshell

Following the correct order of draw and using the right tubes is essential for accurate lab results.

Key Terms

Additive
A substance added to blood collection tubes to preserve or prepare the sample for testing.
Order of Draw
The recommended sequence for filling blood collection tubes to avoid test interference.
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Patient Identification and Special Populations