MCAT Biology › Organic Analyses and Lab Techniques
An alcohol group in a compound would result in a broad dip around what part of the infrared (IR) spectrum?
How many hydrogen peaks appear in the H-NMR spectrum of 3-pentanone?
An alcohol group in a compound would result in a broad dip around what part of the infrared (IR) spectrum?
How many unique peaks would one expect to see on an 1H-NMR reading of the compound shown above?
How many hydrogen peaks appear in the H-NMR spectrum of 3-pentanone?
How many unique peaks would one expect to see on an 1H-NMR reading of the compound shown above?
According to HNMR spectroscopy, which of the following molecules would result in a peak at 9.5ppm?
According to HNMR spectroscopy, which of the following molecules would result in a peak at 9.5ppm?
To isolate DNA from a cell pellet, a student is performing an extraction using phenol and chloroform. The cells were lysed with 0.5mL of lysis buffer. 0.5mL extraction solution, consisting of phenol:chloroform:isoamyl alcohol (25:24:1), was added to the lysate. The sample was centrifuged at high speed for five minutes. The liquid in the tube was separated into three phases after centrifugation (see diagram).
In which layer will nucleic acids (RNA and DNA) be found?
To isolate DNA from a cell pellet, a student is performing an extraction using phenol and chloroform. The cells were lysed with 0.5mL of lysis buffer. 0.5mL extraction solution, consisting of phenol:chloroform:isoamyl alcohol (25:24:1), was added to the lysate. The sample was centrifuged at high speed for five minutes. The liquid in the tube was separated into three phases after centrifugation (see diagram).
In which layer will nucleic acids (RNA and DNA) be found?