Organic Analyses and Lab Techniques

Practice Questions

MCAT Biology › Organic Analyses and Lab Techniques

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1

An alcohol group in a compound would result in a broad dip around what part of the infrared (IR) spectrum?

2

How many hydrogen peaks appear in the H-NMR spectrum of 3-pentanone?

3

An alcohol group in a compound would result in a broad dip around what part of the infrared (IR) spectrum?

4

Q2

How many unique peaks would one expect to see on an 1H-NMR reading of the compound shown above?

5

How many hydrogen peaks appear in the H-NMR spectrum of 3-pentanone?

6

Q2

How many unique peaks would one expect to see on an 1H-NMR reading of the compound shown above?

7

According to HNMR spectroscopy, which of the following molecules would result in a peak at 9.5ppm?

8

According to HNMR spectroscopy, which of the following molecules would result in a peak at 9.5ppm?

9

To isolate DNA from a cell pellet, a student is performing an extraction using phenol and chloroform. The cells were lysed with 0.5mL of lysis buffer. 0.5mL extraction solution, consisting of phenol:chloroform:isoamyl alcohol (25:24:1), was added to the lysate. The sample was centrifuged at high speed for five minutes. The liquid in the tube was separated into three phases after centrifugation (see diagram).

Phenol_chloroform

In which layer will nucleic acids (RNA and DNA) be found?

10

To isolate DNA from a cell pellet, a student is performing an extraction using phenol and chloroform. The cells were lysed with 0.5mL of lysis buffer. 0.5mL extraction solution, consisting of phenol:chloroform:isoamyl alcohol (25:24:1), was added to the lysate. The sample was centrifuged at high speed for five minutes. The liquid in the tube was separated into three phases after centrifugation (see diagram).

Phenol_chloroform

In which layer will nucleic acids (RNA and DNA) be found?

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