Which of the following will be the complimentary pairing of this coded gene during transcription?

3’ GCTAGC 5’

If a gene has a sequence of 5'-AGCTGCCTT-3', what would be the complementary mRNA sequence that leaves the nucleus to be translated?

An important part of creating DNA primers when performing a PCR or other quantitative analysis is the melting point of the primer. Which set of primers would most likely work well together as the forward and reverse primers of a PCR?

The codons GGU, GGA, GGC, and GGG all code for the same amino acid, glycine. What biological term is used to describe this phenomenon?

Which segment of DNA would have the highest melting point when paired with its complimentary strand?

Which of the following correctly arranges the bases on the anti-codon loop of a tRNA carrying tryptophan?

Tryptophan codon: 3'-UGG-5'

Which of the following options include degenerate codons?

The base sequence of one strand of tRNA is v. What is the corresponding sequence of DNA?

You are a student researcher cloning a gene that is around 1500 bases long into a vector for recombinant expression. Starting with cDNA, you succesfully clone and transfect bacterial cells to propogate the plasmid. You sequence the plasmid to check and make sure that the target gene has been succesfully incoorporated into the vector. After checking the sequence, you notice that there is a single nucleotide that has been switched from an A to a G several hundred basepairs after the start codon. You express the protein anyway. After subjecting the protein to SDS-page, you learn that it exists at its expected length, but in a functional asssay, the protein seems to have lost its function. What type of mutation is this called?

In 2013, scientists linked a cellular response called the unfolded protein response (UPR) to a series of neurodegenerative diseases, including such major health issues as Parkinson’s and Alzheimer’s Disease. According to their work, the unfolded protein response is a reduction in translation as a result of a series of enzymes that modify a translation initiation factor, eIF2, as below:

In the above sequence, the unfolded protein sensor binds to unfolded protein, such as the pathogenic amyloid-beta found in the brains of Alzheimer’s Disease patients. This sensor then phosphorylates PERK, or protein kinase RNA-like endoplasmic reticulum kinase. This leads to downstream effects on eIF2, inhibition of which represses translation. It is thought that symptoms of neurodegenerative disease may be a result of this reduced translation.

During translation, the genetic code is used to convert a sequence of nitrogenous bases in mRNA to an amino acid sequence. Which of the following is true of the genetic code?

I. More than one codon sequence codes for a single amino acid

II. The most 5' position of the codon on mRNA is the wobble position

III. Each codon sequence only codes for one amino acid