If a gene has a sequence of 5'-AGCTGCCTT-3', what would be the complementary mRNA sequence that leaves the nucleus to be translated?

Prions are the suspected cause of a wide variety of neurodegenerative diseases in mammals. According to prevailing theory, prions are infectious particles made only of protein and found in high concentrations in the brains of infected animals. All mammals produce normal prion protein, PrPC, a transmembrane protein whose function remains unclear.

Infectious prions, PrPRes, induce conformational changes in the existing PrPC proteins according to the following reaction:

PrPC + PrPRes → PrPRes + PrPRes

The PrPRes is then suspected to accumulate in the nervous tissue of infected patients and cause disease. This model of transmission generates replicated proteins, but does so bypassing the standard model of the central dogma of molecular biology. Transcription and translation apparently do not play a role in this replication process.

This theory is a major departure from previously established biological dogma. A scientist decides to test the protein-only theory of prion propagation. He establishes his experiment as follows:

Homogenized brain matter of infected rabbits is injected into the brains of healthy rabbits, as per the following table:

Rabbit 1 and 2: injected with normal saline on days 1 and 2

The above trials serve as controls.

Rabbit 3 and 4: injected with homogenized brain matter on days 1 and 2

The above trials use unmodified brain matter.

Rabbit 5 and 6: injected with irradiated homogenized brain matter on days 1 and 2

The above trials use brain matter that has been irradiated to destroy nucleic acids in the homogenate.

Rabbit 7 and 8: injected with protein-free centrifuged homogenized brain matter on days 1 and 2

The above trials use brain matter that has been centrifuged to generate a protein-free homogenate and a protein-rich homogenate based on molecular weight.

Rabbit 9 and 10: injected with boiled homogenized brain matter on days 1 and 2

The above trials use brain matter that have been boiled to destroy any bacterial contaminants in the homogenate.

Which intermediates of the central dogma of molecular biology below are present in normal cellular replication, but apparently absent in the above model of prion replication?

I. mRNA

II. tRNA

III. Protein

Which of the following will be the complimentary pairing of this coded gene during transcription?

3’ GCTAGC 5’

Which of the following experimental methods cannot be used to measure the relative abundance of a particular mRNA?

Prions are the suspected cause of a wide variety of neurodegenerative diseases in mammals. According to prevailing theory, prions are infectious particles made only of protein and found in high concentrations in the brains of infected animals. All mammals produce normal prion protein, PrPC, a transmembrane protein whose function remains unclear.

Infectious prions, PrPRes, induce conformational changes in the existing PrPC proteins according to the following reaction:

PrPC + PrPRes → PrPRes + PrPRes

The PrPRes is then suspected to accumulate in the nervous tissue of infected patients and cause disease. This model of transmission generates replicated proteins, but does so bypassing the standard model of the central dogma of molecular biology. Transcription and translation apparently do not play a role in this replication process.

This theory is a major departure from previously established biological dogma. A scientist decides to test the protein-only theory of prion propagation. He establishes his experiment as follows:

Homogenized brain matter of infected rabbits is injected into the brains of healthy rabbits, as per the following table:

Rabbit 1 and 2: injected with normal saline on days 1 and 2

The above trials serve as controls.

Rabbit 3 and 4: injected with homogenized brain matter on days 1 and 2

The above trials use unmodified brain matter.

Rabbit 5 and 6: injected with irradiated homogenized brain matter on days 1 and 2

The above trials use brain matter that has been irradiated to destroy nucleic acids in the homogenate.

Rabbit 7 and 8: injected with protein-free centrifuged homogenized brain matter on days 1 and 2

The above trials use brain matter that has been centrifuged to generate a protein-free homogenate and a protein-rich homogenate based on molecular weight.

Rabbit 9 and 10: injected with boiled homogenized brain matter on days 1 and 2

The above trials use brain matter that have been boiled to destroy any bacterial contaminants in the homogenate.

Which intermediates of the central dogma of molecular biology below are present in normal cellular replication, but apparently absent in the above model of prion replication?

I. mRNA

II. tRNA

III. Protein

An important part of creating DNA primers when performing a PCR or other quantitative analysis is the melting point of the primer. Which set of primers would most likely work well together as the forward and reverse primers of a PCR?

Which of the following will be the complimentary pairing of this coded gene during transcription?

3’ GCTAGC 5’

If a gene has a sequence of 5'-AGCTGCCTT-3', what would be the complementary mRNA sequence that leaves the nucleus to be translated?

Which of the following experimental methods cannot be used to measure the relative abundance of a particular mRNA?

An important part of creating DNA primers when performing a PCR or other quantitative analysis is the melting point of the primer. Which set of primers would most likely work well together as the forward and reverse primers of a PCR?