Cell Structures and Organelles - High School Biology

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Question

Where does the Krebs cycle take place in the mitochondria?

Answer

The mitochondria are the site for aerobic respiration in the cell. Both the Krebs cycle and electron transport chain are found in the mitochondria, while glycolysis (anaerobic metabolism) takes place in the cytoplasm. The Krebs cycle takes place in the mitochondrial matrix, where pyruvate is used to generate NADH and FADH2. These molecules are then taken to the inner mitochondrial membrane, where the electron transport chain is located. The electron transport chain pushes protons into the intermembrane space, creating the proton gradient that fuels ATP synthesis.

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Question

What organelle is associated with photosynthesis in algae?

Answer

Chloroplasts are an organelle that harbors large amounts of the green pigment chlorophyll. The chloroplast converts the energy of sunlight into chemical energy through a process called photosynthesis. This takes place inside a system of membranous sacs called thylakoids within the chloroplast.

Plants, algae, and cyanobacteria all produce energy via photosynthesis. Plants and algae use chloroplasts, but cyanobacteria are prokaryotes and do not contain membrane-bound organelles. Cyanobacteria use internal thylakoid structures to perform photosynthesis.

Plasmids are circular pieces of DNA found in prokaryotes, and are not linked to photosynthesis. Guard cells are responsible for opening and closing stomata on the surface of plant leaves, and are also not involved in photosynthesis.

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Question

A researcher analyzes the effects of a particular disease that affects the eye. He observes that the genes responsible for this disease are located on the mitochondria. What can the researcher conclude about this disease?

Answer

Mitochondria are unique organelles because they contain their own double-stranded DNA. The genes on these DNA molecules are always transmitted to the offspring from the mother. During development, the mitochondrial DNA from the sperm (the gamete from father) is destroyed in the embryo. Since all genes in the mitochondria are only inherited from the mother, we can conclude that this disease is maternally inherited. We also know that the genes must be found on double-stranded DNA. There are very small amounts of double-stranded RNA molecules inside mitochondria, but they don’t code for any known diseases.

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Question

Which of the following is true regarding mitochondria?

Answer

Mitochondria are the site of Krebs cycle and oxidative phosphorylation; however, they are not the site of glycolysis. Glycolysis occurs in the cytosol. Mitochondria have two membranes: the inner and the outer membrane. The space between these two membranes is termed the intermembrane space. The mitochondrial matrix is inside the inner membrane and is the site of Krebs cycle. Scientists theorize that mitochondria and chloroplasts (from plants) were initially prokaryotic organisms that joined with another cell to form a symbiotic relationship, not a parasitic relationship. This theory is called the endosymbiotic theory and it explains the reasons why mitochondria contain DNA.

Mitochondria do have their own ribosomes, which produce unique mitochondrial proteins. Remember, however, that the mitochondria still utilize the normal proteins produced by the ribosomes in the rough endoplasmic reticulum and cytosol.

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Question

The mitochondria are commonly considered the powerhouses of the cell. The energy (ATP) necessary for cell function is created within the mitochondria via the process of oxidative phosphorylation (the electron transport chain). In order for oxidative phosphorylation to be effective, certain proteins must maintain a proton gradient between the two membranes that surround the mitochondria.

Studying the structure of the mitochondria, you will notice that the organelle has two membranes. The outer membrane is smooth, while the inner membrane has numerous inversions and folds. These folds are called cristae. What is the purpose of the cristae?

Answer

The cristae increase the surface area available within the cell. In order for oxidative phosphorylation of occur, certain proteins must be embedded in the inner mitochondrial membrane. The fold in the membrane allow for a larger surface area, which in turn allows more of these proteins to be embedded. More proteins to create energy means more energy (ATP) for the cell.

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Question

Which of the following is not true regarding mitochondria and chloroplasts?

Answer

Mitochondria and chloroplasts are plastids, and are thought to have arisen in eukaryotic cells via endosymbiosis. All the other options are true of mitochondria and chloroplasts.

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Question

Which of the following organelles is commonly referred to as the powerhouse of the cell?

Answer

The mitochondria are responsible for synthesis of the majority of the cell's ATP. This is why mitochondria are commonly referred to as the powerhouse of the cell. The rough endoplasmic reticulum and Golgi apparatus are involved in protein synthesis and delivery. The nucleus houses the DNA and is the site of transcription and ribosome assembly (nucleolus). The lysosome contains hydrolytic enzymes that are used for degradation of certain materials.

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Question

Which of the following organelles contain their “own” DNA?

Answer

All of the listed organelles contain DNA. Scientists have identified nuclear DNA, mitochondrial DNA and chloroplast DNA that are exclusive to these cellular structures.

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Question

Which organelle's main function is the conversion of the potential energy of food molecules into ATP?

Answer

Mitochondria is the organelle involved in ATP production. Specifically, the Krebs cycle and electron transport chain occurs in the mitochondria. Smooth ER synthesizes lipids and detoxifies harmful substances. Rough ER and ribosomes are involved in protein synthesis.

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Question

All of the following are true of chloroplasts except __________.

Answer

Chloroplasts are the organelles found in plant cells that contain chlorophyll pigments which conduct photosynthesis in plants , some bacteria, and some protists. They form glucose molecules, which can be connected to form polysaccharides of starch. Animal cells do not have chloroplasts. Rather, animals need to eat their food, they cannot produce their own food from the sun.

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Question

Which of the following cellular organelles is responsible for making a majority of the cell's energy?

Answer

Mitochondria produces most of the energy of the cell. The nucleus stores genetic information and is the site of transcription. The rough endoplasmic reticulum is the site of translation of proteins that have destinations other than in the cytoplasm. These proteins made in the rough endoplasmic reticulum are subsequently transported to the Golgi apparatus, where they are modified, packaged, and sent to their final destinations. Lysosomes have an acidic environment in which digestive enzymes break down polymers that will eventually be recycled.

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Question

How is mitochondrial DNA (mDNA) different from nuclear DNA?

Answer

Mitochondrial DNA is much smaller and simpler than nuclear DNA, meaning it does not need histones or introns. Mitochondrial DNA is also circular, like bacterial DNA, rather than linear like nuclear DNA. As the ovum is the only contributor of mitochondria to an offspring, all mDNA must therefore be inherited from the mother.

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Question

What is the "powerhouse" of the cell?

Answer

The mitochondria is responsible for making the energy for the cell by cellular respiration. It does this by taking the major breakdown products of glucose (pyruvate and NADH) and converting them into ATP by the citric acid cycle and electron transport chain. This is an oxygen-dependent process.

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Question

Which of the following organelles has only one membrane?

Answer

Lysosomes are specialized vesicles that bud off of the Golgi apparatus and house digestive enzymes within the cell. Lysosomes have only one outer membrane, made of a phospholipid bilayer, similar to the plasma membrane surrounding the cell.

In contrast, the nucleus, mitochondria, and chloroplasts all have two outer membranes. Each of these is made of a lipid bilayer, for a total of four layers of phospholipids around these structures.

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Question

Translation is a process that creates protein from mRNA. Where does translation occur in a eukaryotic cell?

Answer

Recall that the central dogma of molecular biology states that the information flow within a cell involves DNA replication, transcription, and translation. DNA replication involves the formation of two identical DNA molecules from a single molecule and transcription involves synthesis of mRNA from DNA. The DNA molecules can’t leave the nucleus, therefore, both of these processes occur in the nucleus

Translation involves the synthesis of proteins from mRNA molecules. The mRNA molecules exit the nucleus after transcription (through nuclear pores) and enter the cytosol. The ribosomes in the cytosol bind to mRNA and facilitate translation; therefore, translation occurs in the cytosol.

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Question

Which of the following structures is not made up of tubulin?

I. Flagella

II. Mitotic spindle

III. Microfilament

Answer

A cell’s cytoskeleton is made up of three types of filaments: microfilaments, intermediate filaments, and microtubules. Microfilaments are made up of actin, whereas microtubules are made up of tubulin. Flagella, cilia, and the mitotic spindle of the cell are made from microtubules. The only filament given that is not made up of tubulin is microfilament.

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Question

The lumen of which of the following organelles contains the highest concentration of hydrogen ions?

Answer

The question states that the lumen of the organelle contains a high concentration of hydrogen ions. Recall that a high concentration of hydrogen ions results in a low pH. Lysosomes are organelles that contain degradative, hydrolytic enzymes that break down the cell’s waste products. These enzymes need a low pH to be functional, therefore, the lumen of lysosomes has a low pH (around a pH of 5). Lysosomes are known for having a high concentration of hydrogen ions to facilitate this acidic environment.

The other organelle known for sequestering hydrogen ions is the mitochondria. In mitochondria, hydrogen ions are used to create the gradient in the intermembrane space that is used to make ATP.

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Question

Which of the following sequences represents an accurate order of size, from smallest to largest?

Answer

Atoms are the smallest functional unit of matter and are used to construct complex molecules, such as proteins. A protein is composed of numerous smaller atoms.

Ribosomes are composed of ribosomal RNA (rRNA) and proteins, but are smaller than viruses. Viruses are composed of protein coats that house genetic information coded in nucleic acids.

Mitochondria are membrane-bound organelles within the cell. These structures are smaller than the nucleus, but larger than ribosomes. While viruses must be small enough to enter the nucleus through nuclear pores, mitochondria are too large to travel much within the cell.

Cells are the basic functional unit of life. Prokaryotic cells and viruses are both significantly smaller than eukaryotic cells. Eukaryotic cells are relatively large in order to accommodate the various organelles and process that occur within them.

In order from smallest to largest, the answer is atoms, proteins, ribosomes, viruses, mitochondria, a eukaryotic cell.

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Question

What is autophagy?

Answer

Eukaryotic cells are very resourceful in every way they function. When a cell part becomes worn out or non-functional, these part is broken down and recycled by the cell via lysosomal activation. This process is known as autophagy. The biological monomers produced from the catabolic process can then be used to build new polymers and macromolecules.

Exocytosis is the process by which contents from within the cell are expelled across the membrane. This generally occurs to assist cell signaling, such as the exocytosis of neurotransmitters. Positive selection is the process by which T-lymphocytes are presented by "self" antigens. If the T-cell reacts to the "self" antigen, it is destroyed to prevent autoimmune reactions.

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Question

Which of the following correctly describes the function of a lysosome?

Answer

Lysosomes are membrane-bound organelles in the cell with a low pH internal environment. This low pH environment enables the activity of certain enzymes that reside in the lysosomes. These enzymes, known as hydrolytic enxymes, break down damaged or inactive proteins.

The incorrect answer choices largely outline processes accomplished by other organelles. The smooth endoplasmic reticulum synthesizes lipids. The Golgi apparatus and rough endoplasmic reticulum are responsible for protein modification and packaging prior to export from the cell; frequently, this will include addition of different monomers, such as a monosaccharide or lipid to adjust the protein's identity or function. Transportation of molecules through the cytoplasm is accomplished by the cytoskeleton and microtubule system.

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