Understanding Operons and Promoters - GRE Subject Test: Biology

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Question

Which of the following accurately describes the promoter?

Answer

The promoter is a specific segment of DNA that signals the starting point of transcription. RNA polymerase attaches to the promoter and proceeds to create the mRNA primary transcript.

DNA polymerase binds to the RNA primer to begin DNA replication. Ribosomes bind to the 5' cap on eukaryotic mRNA.

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Question

The lac operon is typically found in prokaryotes in order to utilize lactose in the event that glucose is absent. How does the presence of lactose affect the lac operon?

Answer

The lac operon is set up in a way so that the lac repressor is able to be transcribed, regardless of glucose and lactose levels. The lac repressor will then attach to the operator, which inhibits transcription. If lactose is present, it will bind to the lac repressor, and make it detach from the operator.

This process allows the operon to be transcribed in the event that glucose is absent. If glucose is absent, but lactose is not present, then the repressor will remain in place and transcription will not take place.

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Question

Which conditions would result in the largest levels of lac operon transcription?

Answer

The important thing to remember about the lac operon is that it is transcribed when glucose is absent from the cell, but lactose is present and can be utilized. As a result, the operon's transcription would be high if there are both high levels of lactose available, and very little amounts of glucose.

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