Dicer is an endonuclease that cleaves __________ during the process of RNA-interference.

Which protein, associated with the RNA-inducing silencing complex (RISC), activates and cleaves mRNA in RNAi?

Many strains of the model organism Drosophila have been engineered to express RNAi transgenes; that is, these transgenes express a moiety that is capable of targeting specific mRNAs to be degraded. This effectively results in downregulation of certain genes, permitting scientists to study how loss of that gene effects the organism.

What is the structure of the RNAi moiety that targets and binds to specific mRNAs?

Many strains of the model organism Drosophila have been engineered to express RNAi transgenes; that is, these transgenes express a moiety that is capable of targeting specific mRNAs to be degraded. This effectively results in downregulation of certain genes, permitting scientists to study how loss of that gene effects the organism.

Consider the following hypothetical situation. Gene A codes for the protein Enzyme A; that is, Gene A is expressed and is translated to make Enzyme A. You obtain a fly expressing an RNAi that targets Gene A's mRNA. If you express this RNAi in a fly (Drosophila), which of the following is the most likely result you'd expect if you were able to monitor Enzyme A levels with a fluorescent antibody and compared a wild-type fly to one expressing the RNAi?

Which of the following statements best describes the function of RNAi?

Which of the following techniques could help a researcher inhibit the expression of a target gene?