Which of the following is not a methodology to obtain either transient or stable knockout/ knockdown of a gene?

Genetically altering an animal to reduce the expression of a gene of interest can be a labor intensive process that does not necessarily produce complete loss of a gene. A genetic null is an animal in which the gene has been completely (or nearly completely) excised and as such, no protein is produced. A genetic hypomorph is an animal in which only part of a gene has been deleted and as such, a lower amount of protein or a dysfunctional protein is produced, but it is still there. Hypomorphs can be close to null, perhaps only retaining 5-10% of normal function, or they can be close to wild-type, retaining 80-90% of gene function and having mild mutant phenotypes.

You are studying Gene H, a gene that regulates head size, and the more Gene H is expressed, the larger the organism's head is. You have one animal that is nullfor Gene H, and one that is a hypomorphfor Gene H. You compare the head sizes of these animals. Which of the following results is least likelyto be true from your experiment? You can assume that a wild-type animal will have the largest head size of the three.