GRE Subject Test: Biology › Understanding Cloning and PCR
Which of the following is not a component of a polymerase chain reaction mix?
Which is not a characteristic of a multiplex PCR reaction?
Majority of the time PCR amplifications include an Initialization step, also known as the Hot Start. What is the purpose of the Hot Start?
What are the three steps to PCR thermocycling?
A student researcher wants to clone the human hemoglobin gene (HBB) into an expression vector so that he can express HBB in mouse cells and observe the resulting phenotype.
Which sequence of techniques will allow the student researcher to successfully clone the HBB gene?
A researcher is performing PCR to amplify a sample of DNA. Unfortunately, he forgot to add the DNA primer prior to starting the experiment. Which of the following results is he most likely to observe?
After four cycles of thermocycling, how many copies of the targeted region will be in the PCR product?
Which step of PCR thermocycling is meant to allow the DNA polymerase to add nucleotides to the complementary strand?
What type of instrument is generally used to complete a PCR amplification?
The goal of a PCR reaction is to __________.