Other Lab Techniques

Practice Questions

GRE Subject Test: Biology › Other Lab Techniques

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1

The process by which anatomical specimens are separated and analyzed in a detailed manner is termed __________.

2

The process by which anatomical specimens are separated and analyzed in a detailed manner is termed __________.

3

When would it be appropriate to use extraction in order to separate compounds in a solution?

4

When would it be appropriate to use extraction in order to separate compounds in a solution?

5

Bacterial transformations are a useful tool for biologists to replicate valuable plasmid DNA so that it can be used for a myriad of experiments. A student researcher has designed an experiment to determine the effects of pre-incubation duration, heat shock duration, and heat shock temperature on transformation efficiency. The student transformed 1μg of a β**-**lactamase encoding plasmid and recovered the bacteria for 1 hour at 37°C (shaking at 225rpm) for all conditions in Table 1. He then plated the transformed bacteria on ampicillin agar places and observed growth 24 hours later. Table 1 outlines the variables in the experiment.

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If the student researcher had also done trials with a heat shock temperature of 36°C, the resulting transformation yield would most likely be __________.

6

Bacterial transformations are a useful tool for biologists to replicate valuable plasmid DNA so that it can be used for a myriad of experiments. A student researcher has designed an experiment to determine the effects of pre-incubation duration, heat shock duration, and heat shock temperature on transformation efficiency. The student transformed 1μg of a β**-**lactamase encoding plasmid and recovered the bacteria for 1 hour at 37°C (shaking at 225rpm) for all conditions in Table 1. He then plated the transformed bacteria on ampicillin agar places and observed growth 24 hours later. Table 1 outlines the variables in the experiment.

Untitled

If the student researcher had also done trials with a heat shock temperature of 36°C, the resulting transformation yield would most likely be __________.

7

Which of the following is a method for sample introduction into a capillary electrophoresis system?

8

Which of the following is a method for sample introduction into a capillary electrophoresis system?

9

A student co-immunoprecipitates the NF-kappa B transcription factor that is bound to a protein, which the student cannot identify. What technique would be suitable for identifying this unknown protein?

10

A student co-immunoprecipitates the NF-kappa B transcription factor that is bound to a protein, which the student cannot identify. What technique would be suitable for identifying this unknown protein?

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