Which term describes DNA that has been altered by genes from a different organism, typically from a different species?

Which term describes DNA that has been altered by genes from a different organism, typically from a different species?

Which of the following is not a methodology to obtain either transient or stable knockout/ knockdown of a gene?

Which of the following is not a methodology to obtain either transient or stable knockout/ knockdown of a gene?

A scientist seeks to synthesize a rare human protein in lab. To accomplish this, she utilizes recombinant techniques to insert the DNA of a eukaryotic gene for the protein into bacterial plasmids. These plasmids are transformed into bacteria for expression. She is disappointed to discover that the gene product from the bacteria is not the correct protein. What corrective step could she take in the procedure to fix this issue?

A scientist seeks to synthesize a rare human protein in lab. To accomplish this, she utilizes recombinant techniques to insert the DNA of a eukaryotic gene for the protein into bacterial plasmids. These plasmids are transformed into bacteria for expression. She is disappointed to discover that the gene product from the bacteria is not the correct protein. What corrective step could she take in the procedure to fix this issue?

Dicer is an endonuclease that cleaves __________ during the process of RNA-interference.

Dicer is an endonuclease that cleaves __________ during the process of RNA-interference.

Which protein, associated with the RNA-inducing silencing complex (RISC), activates and cleaves mRNA in RNAi?

Many strains of the model organism Drosophila have been engineered to express RNAi transgenes; that is, these transgenes express a moiety that is capable of targeting specific mRNAs to be degraded. This effectively results in downregulation of certain genes, permitting scientists to study how loss of that gene effects the organism.

What is the structure of the RNAi moiety that targets and binds to specific mRNAs?