Which of the following is a primary factor that dictates how far a protein will migrate during SDS-PAGE?

Which of the following is a primary factor that dictates how far a protein will migrate during SDS-PAGE?

A student researcher overexpresses an exogenous protein in cell culture and wants to determine if that protein, is in fact, overexpressed. What technique would best demonstrate that this protein is expressed in these cells?

A student researcher overexpresses an exogenous protein in cell culture and wants to determine if that protein, is in fact, overexpressed. What technique would best demonstrate that this protein is expressed in these cells?

A cell line was engineered to contain and express a DNA sequence frequently associated with pancreatic cancer, with the hope that the cell line will undergo rapid cell division much like the cancerous cells. You want to test whether or not the cell line is efficiently transcribing this DNA by measuring the quantity of mRNA produced. Which of the following methods would be best suited for testing this?

A cell line was engineered to contain and express a DNA sequence frequently associated with pancreatic cancer, with the hope that the cell line will undergo rapid cell division much like the cancerous cells. You want to test whether or not the cell line is efficiently transcribing this DNA by measuring the quantity of mRNA produced. Which of the following methods would be best suited for testing this?

As opposed to electrophoresis with a more standard agar gel, what does polyacrylamide gel electrophoresis (PAGE) allow for when working with DNA?

As opposed to electrophoresis with a more standard agar gel, what does polyacrylamide gel electrophoresis (PAGE) allow for when working with DNA?

When performing a western blot, what is the purpose of adding a secondary antibody?

When performing a western blot, what is the purpose of adding a secondary antibody?