Which of the following is not a component of a polymerase chain reaction mix?

Which of the following is not a component of a polymerase chain reaction mix?

After a double digest with EcoRI and HindIII, a gel electrophoresis shows that you have several restriction fragment bands. You see bands of lengths 3kb, 4kb and 5kb. From previous tests, you know that there are two EcoRI restriction sites and two HindIII restriction sites in the DNA fragment you are studying. How many restriction fragments are really in your reaction?

Which is not a characteristic of a multiplex PCR reaction?

After a double digest with EcoRI and HindIII, a gel electrophoresis shows that you have several restriction fragment bands. You see bands of lengths 3kb, 4kb and 5kb. From previous tests, you know that there are two EcoRI restriction sites and two HindIII restriction sites in the DNA fragment you are studying. How many restriction fragments are really in your reaction?

Which is not a characteristic of a multiplex PCR reaction?

Majority of the time PCR amplifications include an Initialization step, also known as the Hot Start. What is the purpose of the Hot Start?

Majority of the time PCR amplifications include an Initialization step, also known as the Hot Start. What is the purpose of the Hot Start?

Traditional Sanger style DNA sequencing relies on a method called chain termination. What type of molecule is used to terminate DNA chains to create molecules of all possible lengths covering the fragment to be sequenced?

Traditional Sanger style DNA sequencing relies on a method called chain termination. What type of molecule is used to terminate DNA chains to create molecules of all possible lengths covering the fragment to be sequenced?