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To which of the following structures in a typical molecule of tRNA is an amino acid attached?
Amino acids are attached to the CCA tail of a tRNA. These are found at the 3' end of tRNA molecules and are important for recognition by aminoacyl tRNA synthetases (enzymes that actually attach the amino acids to the tRNA). The anticodon loop, as the name suggests, contains the anticodon, which will be important during translation for recognizing mRNA sequences. The D-loop and the variable loop are other portions of the tRNA that are important for maintaining structure and recognition.
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Chloramphenicol prevents protein translation by which of the following mechanisms?
Tetracycline blocks the binding of aminoacyl tRNA to the A site of the ribosome.
Cyclohexamide blocks the translocation reaction on ribosomes.
Rifamycin blocks the initiation of RNA chains by binding to RNA polymerase.
Chloramphenicol blocks the pepidyl transferase reaction on the ribosome.
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During translation, which site in the ribosome allows for tRNA moelcules to enter the complex?
The ribosomal complex has three sites where tRNA moelcules can be oriented during the process of translation: the A site, the P site, and the E site. During polypeptide elongation, a tRNA with an attached amino acid will enter at the A site. It will then move to the P site, now holding the growing polypeptide chain. All tRNAs no longer holding an amino acid will exit the ribosome at the E site.
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On which of the following molecules could you find an anticodon?
In order to make sure that the proper amino acid is added to the growing polypeptide chain, an anticodon found on the tRNA carrying the amino acid must be a match for the codon found on the mRNA.
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Which of the following most accurately describes the chronological order of ribosome biogenesis in eukaryotes?
Ribosomal proteins are translated in the cytoplasm and rRNA genes are transcribed in the nucleolus. Following protein translation, these proteins enter the nucleus through nuclear pores and localize to the nucleolus. Here, transcribed rRNA associates with the ribosomal proteins to form the 60S and 40S eukaryotic ribosomal subunits. Prokaryotes have 50S and 30S subunits. The ribosomal subunits then translocate to the cytoplasm where they join together to form fully functional ribosomes.
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How many ribosomal binding sites are there and what are their functions?
The correct answer is there are three sites. A site binds aminoacyl-tRNA, P site binds peptidyl-tRNA, E site binds free tRNA before ribosomal exit.
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