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Which of the following techniques would be most useful for a researcher who is trying to determine the structure of a protein?
X-ray crystallography is a very powerful technique used to determine the structure of proteins. It involves first obtaining a crystal of your protein and then shooting x-rays through the sample and observing the resulting diffraction pattern.
Equilibrium centrifugation is a separation technique. FPLC is used to purify proteins. LCMS is used to analyze large samples of peptides quickly.
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What does a microarray allow you to visualize?
A microarray is a chip with many copies of short segments of DNA that allows you to visualize the expression level of potentially unlimited genes simultaneously. It only informs you on how many copies of the gene are present in a sample, and does not give any information on gene sequence/length, etc.
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Which type of microscopy is needed to study subcellular structures like organelles?
Light microscopy can magnify a sample up to 1000X. this is not enough to visualize subcellular structures like organelles in cells. Electron microscopy can magnify a sample much higher than 1000X and so can be used to visualize subcellular structures. Both scanning and transmission electron microscopy magnify to this extent.
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What type of microscopy would you use if you want to get a topographical/3D image of your sample?
Scanning electron microscopy runs a beam of electrons over the surface of a specimen. This beam changes in length as the specimen changes in height off the slide. The microscope can detect the change in beam length and generate a 3D image of the specimen. All of the other forms of microscopy listed produce 2D images.
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Light microscopy and electron microscopy are used for the same purpose: to magnify very small objects for further examination by using radiation. The fundamental difference is that light microscopes use radiation of visible light, and electron microscopes use radiation of electron beams. This technical difference impart different properties on each type of microscopy. Each of the following describes a difference between light and electron microscopy, except __________.
Light microscopy does not need a tungsten coil to produce visible light, only a strong but simple light source is required. Electron microscopy does employ a tungsten coil and magnets for this purpose. Each other answer choice describes a difference between the two types of microscopy.
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Which of the following is true concerning light microscopy?
Light microscopy uses light and a glass lens to magnify images. The higher the magnification, the more blurry the image. Special staining techniques are used to produce images via light microscopy. Electrons are not utilized in light microscopy.
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