Help with Golgi Apparatus Function - GRE Subject Test: Biochemistry, Cell, and Molecular Biology

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Question

What is the primary function of the Golgi apparatus?

Answer

The Golgi apparatus is a complex organelle with many various functions. These functions are primarily dedicated to protein modification, protein packaging, and protein sorting. Some of post-translational modifications that take place in this organelle include glycosylation and phosphorylation. The Golgi apparatus is an important relay in the secretory pathway, as it produces the vesicles that contain most secreted peptides.

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Question

Which of the following GTP-binding proteins is associated with COPI during vesicle assembly and budding in the cis-Golgi?

Answer

The correct answer is ADP ribosylation factors (ARFs). ARFs are active when bound by GTP, and inactive when bound by GDP. The active GTP-bound form of ARF binds the vesicle coat protein 1 (COPI), to faciliate vesicle budding from the cis-Golgi face.

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Question

Each of the following functions are associated with the Golgi apparatus, except __________.

Answer

The Golgi apparatus is considered the post office of the cell; proteins are received, sorted, modified, and sent to their final cellular destination. The proteins required for the electron transport chain are localized to the mitochondria, and are not within the Golgi apparatus, thus this is the correct selection.

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