Help with Enzyme Identification - GRE Subject Test: Biochemistry, Cell, and Molecular Biology

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Question

Which of the following changes cannot be accomplished by an enzyme in a chemical reaction?

Answer

An enzme is a biological catalyst that increases the rate of a reaction. This is accomplished by lowering the activation energy necessary to start the reaction. The equilibrium of the reaction, however, is not affected. This means that enthalpy and entropy are not affected by an enzyme's presence.

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Question

The class of enzymes that break bonds by forming a new double bond or ring structure (rather than by hydrolysis or oxidation) is best characterized as which of the following?

Answer

The correct answer is lyases. This class of enzymes only requires one substrate for the forward reaction.

Ligases catalyze the formation of a bond between two molecules, isomerases rearrange the atoms of a molecule, kinases phosphorylate molecules, and transferases transfer or join functional groups from one molecule to another.

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Question

The third step of glycolysis converts fructose-6-phosphate to fructose-1,6-bisphosphate. What type of enzyme mediates this?

Answer

Kinases are enzyme that catalyze the transfer of a phosphate group from ATP to a substrate molecule. The phosphorylation of fructose-6-phosphate to fructose-1,6-phosphate is mediated by a kinase phosphofructokinase.

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