Help with Separation Techniques

Practice Questions

GRE Subject Test: Biochemistry, Cell, and Molecular Biology › Help with Separation Techniques

Questions
7
1

Which of the following techniques would be most useful to isolate intact ribosomes from cells?

2

__________ is a technique used to pull a protein complex out of a solution by targeting one member of the complex with an antibody.

3

What is the main denaturing agent in a western blot gel that disrupts secondary, tertiary, and quaternary protein structure?

4

A new RNA virus was identified, and the high-throughput sequencing of the genome resulted in two unique contigs. Which method would provide insight into the genome structure of this virus?

5

White blood cells and red bloods cells differ in numerous physical properties, one of which is a significant difference in the density of the two cell types. Red blood cells do not have nuclei, making them much less dense and easy to separate out from white blood cells through simple centrifugation techniques. However, there are numerous white blood cell subtypes with similar densities, presenting a challenge in studying specific white blood cell types as they are more difficult to separate out. We can take advantage of the fact that different white blood cell types express different cell-surface molecules.

Which of the following techniques would be most useful to utilize different cell-surface molecules to separate out different types of white blood cells?

6

Which of these options is an important step in the process of DNA extraction and isolation?

7

Ethidium bromide is commonly used to detect presence of DNA in DNA gels under UV light. What type of bond does ethidium bromide form with double-stranded DNA to emit fluorescence?

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