GRE Subject Test: Biochemistry, Cell, and Molecular Biology › Help with Quantification Techniques
A researcher performs a Bradford assay to determine the quantity of an unknown protein in his sample. The standard protein returns absorbance values of 0.101, 0.204, 0.302, 0.405 for the respective quantities of 10ug, 20ug, 30ug, and 40ug of protein. The unknown sample returns an absorbance value of 0.265. What is the quantity of protein in the unknown sample?
A researcher wants to measure the purity of DNA using a spectrophotometer. What wavelength(s) should he/she consider?
Which of the following techniques would help quantify the amount of protein in a sample?
What is the purpose of a Bradford Assay?
A researcher wants to know the exact concentration of protein in his sample. He estimates the amount of protein to be between and
. Which of the following sets of known protein amounts should he use to set up a standard curve for a Bradford assay?