GRE Subject Test: Biochemistry, Cell, and Molecular Biology › Help with Knockout and Mutation Analyses
A scientist is trying to create a mutant cell line that has a specific non-functional protein. After many different mutagenesis techniques, he is unable to create a cell line with the mutation he desires. Which of the follow might be the reason he cannot generate a mutant for this protein?
Which of the following techniques can be used for mutagenesis?
Which of the following is not a way to disrupt the expression of a specific gene?
A student researcher is trying to identify genes that are turned on by a specific transcription factor. Which of the following techniques can the student use?
In a knockout, what is a chimeric mouse?
When using the CRISPR/Cas9 gene editing technology, what genomic functional element does the Cas9 endonuclease recognize to indicate where it introduces a double stranded break?
Which of the following gene editing technologies is not utilized to programmably alter/mutate specific genomic loci in uni/multicellular organisms?