On a Lineweaver-Burk plot, an inhibited enzyme is shown to have a less negative x-intercept than the uninhibited enzyme, but the y-intercept remains the same. The type of inhibition displayed is __________ and the inhibited reaction has a __________ value.

You have an enzyme solution and you add an inhibitor molecule and observe a marked decrease in enzyme activity. You increase the substrate concentration but this does not lead to any observable increase in enzyme activity. What can you conclude about your inhibitor?

A researcher has designed a new type of inhibitor that binds at the active site of an enzyme. What type of inhibition does this molecule display?

Which of the following choices describes a way to graphically determine the type of inhibition being displayed by an inhibitor?

I. Plot initial reaction rate versus the concentration of substrate for the uninhibited enzyme, and then compare to the inhibited enzyme

II. Plot the inverse of the initial reaction rate versus the inverse of the substrate concentration for the uninhibited enzyme, and then compare to the inhibited enzyme

III. Plot the concentration of the inhibitor versus the concentration of substrate