GRE Subject Test: Biochemistry, Cell, and Molecular Biology › Help with Identification Techniques
The minimum distance between two points that can still be seen as distinct structures is termed __________.
A student researcher runs an agarose gel electrophoresis of a restriction enzyme digested plasmid to generate two fragments of 1200 bp and 3500 bp. The student then stains the gel with ethidium bromide. Which of the following is true about the bands that are seen on the gel?
A student researcher in a lab has just discovered a new species of bacteria. Since very little is known about this species, he decides to execute an experiment to test for peptidoglycan in the outer plasma membrane. He determines that the bacteria does not contain an outer layer of peptidoglycan.
What experiment did the student perform? Is this bacteria virulent?
In a western blot, to what does the secondary antibody bind?
When performing whole-mount immunofluorescence of a specimen, background staining is often a concern with custom generated antibodies. Which of the following is not a way in which to increase specificity of the custom antibody?
Of the following choices, which technique would be the most useful for identifying the binding site of a recently discovered transcription factor?
Northern, Southern, and Western blots, as originally invented by E. M. Southern, are techniques used in different areas of molecular biology. What can you learn from a Northern blot?
A researcher wants to determine if two proteins form a complex in cells. What technique is best suited for this experiment?
A researcher barcodes a library of 3000 plasmids expressing unique growth enzymes and transforms them into bacteria. These 3000 transformed bacteria are then all grown in one large culture of minimal media. Why does the researcher barcode these plasmids?
When is indirect immunofluorescence of sectioned specimens beneficial over whole-mounted specimens?