GRE Subject Test: Biochemistry, Cell, and Molecular Biology › Help with Biochemical Tagging
Researchers often fuse a hemagglutinin (HA) or a FLAG tag to proteins to study their cellular localization and function. What is the purpose of this tag?
Which of the following is not a technique for which biochemical tagging can be used to detect a protein?
A scientist wants to observe the localization of a particular protein within a tissue sample. Which of the following tags would be most useful for accomplishing this task?
Why would EDTA be detrimental to an assay for the detection of a protein that has a 6-His tag?
Which of the following describes information that might be obtained from a successful FRET (fluorescence resonance energy transfer) experiment?
Which of the following is not a consideration when biochemically tagging a protein?
A scientist performs a FRAP (fluorescence recovery after photobleaching) experiment. To do so he labeled a protein with a fluorescent probe. Originally, the fluorescent color was ubiquitous throughout the entire sample. His results indicate that fluorescence did not return to anywhere near the normal level after bleaching his sample. What information can be concluded from this experiment?
Which of the following tags would help a researcher locate a protein in vivo?
I. GFP
II. GST
III. His
IV. myc
Which of the following is not a common biochemical tag that scientists fuse to proteins of interest?
What methods were used in early DNA cloning to generate labeled DNA?