Sugar-Phosphate Groups and Phosphodiester Bonds - Biochemistry

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Question

What type of bonds are found between the DNA sugar hydroxyl groups? What are their corresponding carbon numbers?

Answer

The phosphodiester bonds in DNA occur between the 3' and 5' hydroxyl groups on deoxyribose. (This is related to DNA's 5' to 3' directionality as DNA polymerase can only synthesize DNA by adding nucleotides to the 3' hydroxyl group).

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Question

In DNA, the 5-carbon sugar is attached to the nitrogenous base by a __________.

Answer

The beta-N-glycosidic bond attaches the nitrogen on the purine or pyrimidine base to the 1' anomeric carbon on the deoxyribose sugar. Phosphodiester linkages connect the 3' and 5' sugar hydroxyl groups on adjacent nucleotides.

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Question

Which of the following is not a reason that the anhydride linkage of pyrophosphate stores so much energy?

Answer

Phosphorus on both orthophosphate and pyrophosphate is surrounded by 10 electrons. This does not satisfy the octet rule in either case, so that answer choice is false, and therefore the correct answer. With four negative charges close by, pyrophosphate is very unstable, and releases a lot of energy when cleaved. Finally, the two orthophosphates formed from a single pyrophosphate gain an additional resonance structure.

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Question

What type of bond holds the phosphate group to the sugar group in DNA's backbone?

Answer

The type of bond that holds the phosphate group to the sugar in DNA's backbone is called a phosphodiester bond. Hydrogen bonds connect bases to one another and glycosidic bonds occur between deoxyribose groups and the base groups.

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