Amino terminal - Ala - Lys - Glu - Phe - Phe - Ala - Leu - carboxyl terminal.

If the above primary sequence is cleaved by trypsin, on which amino acid will the new amino terminal be?

If a protein is bonded to ubiquitin, this tells the cell that the protein should be __________.

If a protein is bonded to ubiquitin, this tells the cell that the protein should be __________.

Amino terminal - Ala - Lys - Glu - Phe - Phe - Ala - Leu - carboxyl terminal.

If the above primary sequence is cleaved by trypsin, on which amino acid will the new amino terminal be?

Which of the following proteases would cleave lysine at the carbonyl side?

Which of the following proteases would cleave lysine at the carbonyl side?

HMGCoA reductase (3-hydroxy-3-methyl-glutaryl-CoA reductase) is the rate-limiting enzyme in cholesterol synthesis. Which of the following are true about the ubiquitination of this enzyme?

I. When cholesterol levels in the cell are high, the reductase binds to insulin-induced gene 1 proteins.

II. Binding to insulin induced gene 1 proteins leads to ubiquitination and proteasomal degradation of reductase.

III. Ubiquitination occurs through the binding of the C-terminal glycine of ubiquitin to the amino group of a lysine on the reductase.

IV. The enzyme tagged with ubiquitin is recognized by the proteasome where proteolysis occurs.

HMGCoA reductase (3-hydroxy-3-methyl-glutaryl-CoA reductase) is the rate-limiting enzyme in cholesterol synthesis. Which of the following are true about the ubiquitination of this enzyme?

I. When cholesterol levels in the cell are high, the reductase binds to insulin-induced gene 1 proteins.

II. Binding to insulin induced gene 1 proteins leads to ubiquitination and proteasomal degradation of reductase.

III. Ubiquitination occurs through the binding of the C-terminal glycine of ubiquitin to the amino group of a lysine on the reductase.

IV. The enzyme tagged with ubiquitin is recognized by the proteasome where proteolysis occurs.

What is the result of chymotrypsin being added to the peptide shown?

Gly-Ala-Pro-Tyr-His-Cys-Gly-Phe-Gly-Gly-Asn

What is the result of chymotrypsin being added to the peptide shown?

Gly-Ala-Pro-Tyr-His-Cys-Gly-Phe-Gly-Gly-Asn