Biochemistry - Michaelis-Menten Equation

1

Which of the following is true about the Michaelis constant for any given enzyme?

2

Suppose that an enzyme mixture contains an enzyme with a michaelis constant of $5.0*10^{-6}M$ . If the substrate concentration in this mixture is $4.5*10^{-6}M$ , what is the fractional saturation of this enzyme mixture?

3

Based on Michaelis-Menten enzyme kinetics, if a system has a substrate concentration that is significantly more then the Km, which of the following is a sound inference?

4

An enzyme with a $\textup{K}_{\textup{M}}$ value of $5\textup{ mM}$ has a reaction rate of $200\ \frac{\textup{mmol}}{\textup{min}}$ at a substrate concentration of $0.5 \textup{ mmol}$ . What is the maximum reaction rate that this enzyme can achieve when it is saturated with substrate?

5

Which of the following is a correct statement with regards to an enzyme-catalyzed reaction that obeys Michaelis-Menten kinetics?

6

What is the maximum reaction rate if adding $3\textup{ M}$ of substrate produces a reaction rate of $15\ \frac{\textup{mol}}{\textup{s}}$ ?

$(\textup{K}_\textup{m} = 3\textup{ M})$

7

Which of the following will increase the reaction rate of an enzymatic reaction?

I. Adding a competitive inhibitor

II. Increasing the substrate concentration

III. Decreasing the affinity of substrate to the enzyme

8

For a given enzyme catalyzed reaction, the Michaelis constant is 0.6mM and the substrate concentration is 1.0mM. What is the fractional saturation of the enzyme under these conditions?